DR. ΣΑΒΒΑΣ ΑΝΑΣΤΑΣΙΑΔΗΣ: Ο ΕΦΕΥΡΕΤΗΣ ΤΟΥ ECOPLANT ΚΑΙ ΤΟ ΚΑΘΗΓΗΤΙΚΟ ΚΑΤΕΣΤΗΜΕΝΟ ΠΟΥ ΧΑΡΑΚΤΗΡΙΣΕ "ΑΝΕΚΔΟΤΟ" ΤΟ ΒΙΟΓΡΑΦΙΚΟ ΠΟΥ ΔΗΜΟΣΙΕΥΟΥΜΕ.
(ΜΕΡΟΣ ΠΡΩΤΟ)
Είναι εντελώς κοινότυπο αλλά δεν παύει να αντιπροσωπεύει 100% την ωμή αλήθεια ότι κάποιοι στην Ελλάδα που κατέχουν τη δύναμη και τις στρογγυλές σφραγίδες, μπορούν στην κυριολεξία να εξαφανίσουν κάποιους άλλους διακεκριμένους συμπολίτες μας οι οποίοι γύρισαν την πλάτη στα οφίτσια και τις θέσεις-κλειδιά, για να αφοσιωθούν αποκλειστικά στην επιστήμη.
Η περίπτωση του Σάββα Αναστασιάδη, Δόκτορος της Μοριακής Βιοτεχνολογίας είναι χαρακτηριστική: Με τεράστια διεθνή αναγνώριση από τις ΗΠΑ μέχρι την Ινδία και από τη Ρωσία μέχρι τη Ν. Αφρική, ο συγκεκριμένος επιστήμονας δεν μπορεί να εξασφαλίσει μία μόνιμη θέση στο Πανεπιστήμιο της Ξάνθης γιατί προφανώς το εντεταλμένο συμβούλιο των κριτών του δεν συμφωνεί με την εικόνα ενός καθηγητή ο οποίος "κοιμάται και διανυκτερεύει στα θρανία της σχολής", τη στιγμή που -πιθανότατα- άλλοι συνάδελφοί του συνεστιάζονται σε in εστιατόρια και bar κάνοντας δημόσιες σχέσεις με την εκάστοτε εξουσία.
Οι συγκεκριμένοι Κύριοι και Κυρίες αποκάλεσαν το βιογραφικό που παραθέτω πιο κάτω στο 1ο μέρος της έρευνάς μου για την περίπτωση του κ. Αναστασιάδη ως "ανέκδοτο" και προσπαθούν με κάθε τρόπο να στερήσουν αυτό που δίνει ζωή σε κάθε άνθρωπο της επιστήμης: την Έδρα. Δηλαδή το Ιερό της Γνώσης που είναι η απαραίτητη κινητήρια δύναμη κάθε Γενιάς που σέβεται την ιστορία των προγόνων της και κοιτάζει το μέλλον με αισιοδοξία.
Με το θέμα θα ασχοληθώ εκτεταμένα, μέχρι να δικαιωθεί ο Σάββας Αναστασιάδης, ο οποίος έχει υποβάλλει και σχετική μήνυση την οποία θα δημοσιεύσω προσεχώς. Σήμερα δημοσιεύω το βιογραφικό του σε κοινή θέα για να μάθει ο κόσμος ότι σ' αυτό τον τόπο υπάρχουν ΚΑΙ ΘΑ ΥΠΑΡΧΟΥΝ μυαλά που δεν ξέρουν τι θα πει αδιέξοδο και αντιμετωπίζουν κάθε πρόβλημα σαν πρόκληση και ευκαιρία απόδειξης της ανωτερότητας του Ελληνικού Πνεύματος. Ενός Πνεύματος που βολεύει ορισμένους αντί να το προωθούν στη βιτρίνα να το κρύβουν στα αραχνιασμένα υπόγεια της μιζέριας τους.
Απόστολος Κολοβός
Ανεξάρτητος Δημοσιογράφος
CV of Dr. Savas G.
Anastassiadis
Pythia Institute of
Biotechnology
(Owner - Director) Tel. 0035974681575, Mobile 00359895761696
Research in
Biotechnology Co. Vat#. 108851559
Avgi Sohos, 57002 Thessaloniki, Greece, 00359895761696 (BG)
Assistant Professor at Democritus University of Thrace
Phone/Fax: +30 (2395) 051470, Phone: +30-23950-51324
Mobile: +30-6973-801395, 6983-375287, 6955-580977
www.pythia-biotechnology.com Vat# BG202174635
E-mail: sanastassiadis26@gmail.com, sanasta@env.duth.gr
http://www.researchgate.net/profile/Savas_Anastassiadis/
Education and
Scientific Experience
10/2000 to present (Lecturer, C1 Professor, at the present
Assistant C2 Professor) – Research and Teaching at the Environmental Engineering Department of Democritus University of Thrace (Xanthi, Greece).
· I acted as a consultant for the Greek Government
during the EU presidency.
· I started a personal company nam.ed Research in Biotechnology Vat#. 108851559 (Greece).
· Grounder and Owner of Pythia
Institute of Biotechnology (Large
buildings of
former Preliminary School, Avgi, Thessaloniki, Greece)
– Research and
Development activities and pilot scale production (Novel conditioner EcoPlant).
· Grounder of a subsidiary of Pythia Institute of Biotechnology
(Sandanski, Bulgaria,
Vat# BG202174635) – Production company (in near future)
· Research and Development cooperation and publishing
with the Institute
of
Biochemistry and Physiology of
Microorganisms of the Russian Academy of Sciences (Pushchino, Moscow Region, Russia).
· Co-Instructor of Diploma and Ph.D. students
as Visitor Professor at the Institute of Biochemistry and Physiology
of Microorganisms of the Russian Academy of Sciences (Pushchino, Moscow Region, Russia).
01/2000 - 07/2000 Serving in the Greek Army (Poros and Navy Hospital of Salamina).
10/1998 - 10/1999 Postdoctoral Research associate at Tufts University, Biotechnol.
Center (USA)
09/1996 – 12/1998 Senior researcher at Cargill and
ThermoFibergen (ThermoElectron) (USA)
03/1994 - 08/1996 Postdoctoral Research Associate,
Massachusetts Institute of Technology (MIT), Department of Chemical Engineering (USA).
Academic advisor: Bayer Professor Dr. Gregory Stephanopoulos
01/1990 - 04/1994 Dr. Nat. Sc. (Ph.D.
Thesis): Biotechnology Research Institute, Research Center Jülich (RCJ), Germany (former Nuclear Research Center Jülich,
KFA, Germany) and University of Münster (Germany).
Topic of the Ph.D. work: "Continuous Production of Citric acid
and Gluconic
acid with yeast and yeast-like Microorganisms"
Academic Supervisors:
Prof. Dr. Hans-Jürgen Rehm (University of Münster, Germany)
Prof. Dr. Christian Wandrey (University of Bonn and
KFA Jülich)
Ph.D. thesis was financed by Haarmann & Reimer, a daughter company of the
company Bayer, Leverkusen, Germany
1988 - 1989 Master Thesis (Diplomarbeit): "Microbial Events at the Soaking of
Soybeans"
Institute of Molecular Microbiology and
Biotechnology (former Institute of
Microbiology), University of Münster, Germany.
Academic Supervisor: Prof. Dr. Hans-Jürgen Rehm
1982 - 1988 M.S. in Biology (Diploma), University of Münster, Germany
Major: Microbiology
Minor: Biochemistry and Molecular and General Biology
1981 - 1982 Münster Junior College, Münster, Germany
2
1973 - 1979 Sochos High School, Thessaloniki, Greece
1967 - 1973 Preliminary School, Avgi and Thessaloniki, Greece
Research in Industry
09/1996 – 07/1997 Work on contract basis for an agency
(on assignment, Lab
Support Co.), including
4 months in a laboratory in the industry (CARGILL, Minneapolis,
USA). The project focused on the continuous production of
citric acid by yeast
strains of Yarrowia lipolytica (ATCC strains) in chemostat. According to
biotechnology books and bibliography, it would have
been very difficult to
develop an economic commercial continuous production
of citric acid. The
obtained results of up to 190 g/l of continuously
produced citric acids surpass
the production of current industrial batch and
fed-batch fermentations by
Aspergillus niger. Continuous fermentation ran for more
than 40 days.
02/1997 - 12/1998 Work as scientific consultant and
researcher at Thermo
Fibergen Inc., a Thermo
Electron Company (Boston, USA).
I organized and set up the laboratory for fermentation
and microbiological
research and development. I tested various renewable
carbon sources as
substrate for microbial growth and production and I
developed and optimized
new fermentation processes for the continuous
production of organic acids by
new microorganisms, which have very important
applications for environmental
and other industrial purposes (biodegradable plastics) More than 110 g/l of Llactic
acid have been produced continuously with a yield of
about 90% at very
low residence times. Volumetric productivities of up
to 16 g/(l h) and 140 g/l of
lactic acid have been achieved. Furthermore, I
developed and optimized new
fermentation processes for the continuous production
of a novel
ecological noncorrosive biodegradable deicer.
The continuous process was running more than nine
months without any
problems. Compared with existing industrial processes,
the new developed
continuous processes have many advantages and are very
promising for an
industrial production. They base on free growing cells
in chemostat and no
biomass retention is necessary. Biomass retention can
increase the volumetric
productivity. Cheap carbon sources, such as molasses
and glucose derived from
paper sludge have been used successfully (patent
application).
Traditional fermentations for the production of lactic
acid use homofermentative
lactic acid bacteria, which require complex media and
are sensitive to high
osmotic pressures and stress conditions. Existing
industrial fermentation
processes for organic acids are generally batch or fed
batch, because a stable
continuous process is very complicated and difficult
to develop.
Furthermore, I did preliminary theoretical consulting
work for the fermentation of recombinant proteins.
During my studies at the University of Münster
(Germany), I also had visited many special laboratory courses in molecular
biology, immunology, Genetics, Protozoa, Biochemistry etc. Because of my
longtime experiences in research and teaching, I can also start working
successfully with other scientists in new research areas, such as Molecular biology, Genetics, and Fermentation of cell
cultures and
recombinant proteins.
2003-Today Research and Development and
production at Pythia Institute of
Biotechnology
Citric acid production by yeasts from glycerol and
other carbon sources
Gluconic acid production by yeast-like fungi Microbial
production of cellulose from atmospheric CO2
Production
and distribution of a novel soil conditioner named EcoPlant
L-Lysine fermentation
Bioethanol
production
Special Recognition
and achievements
· Received two recognition bonus-awards for outstanding
achievements and innovative activities (patenting) from Nuclear Research Center Jülich (KFA) in Germany (6 patents, Ph.D. thesis).
· Financing of my Ph.D. thesis at the RCJ (KFA) by
Haarmann and Reimer, a Bayer
co. (1990-1994).
· Postdoctoral Research Associate at MIT and Tufts
University (USA).
· Inclusion of biographical profile in 2006-2007 Edition of Who’s Who in Science and Engineering because of the reference value of
outstanding achievements (chronicle of lives and career of the world’s most accomplished scientists and engineers).
· Cooperation and affiliation with the Institute of Biochemistry and Physiology
of Microorganisms and
with the Institute
of theoretical and experimental biophysics of the Russian Academy of Sciences (Publishing, Research and Development) (2005 - present)
· Consulting for the Greek Government in the area of biotechnology (Future developments and possibilities) during the Greek European Presidency (2002-2003).
· I was elected and received a grant (44000 Euro) for
innovative technologies (patents) from the Greek General Secretary of Research and
Technology (G.G.E.T.),
which was accomplished successfully
(2003-2006).
· Invitation from the Russian Academy of Sciences for participating with POSTER
PRESENTATION at the AT THE FOURTH CONGRESS OF RUSSIAN Y.A.
OVCHINNIKOV SOCIETY OF BIOTECHNOLOGISTS, Pushchino
Scientific Centre, Russia
(December 6-7, 2006).
· Member of the RUSSIAN Y.A. OVCHINNIKOV SOCIETY OF BIOTECHNOLOGISTS
· Positive comments from leading International Journals and
scientists
· Reviewer of significant research and
review articles at leading International Journals
· Member of the Editorial Advisory Board in the Journal Recent Patents
on Biotechnology (Bentham Science Publisher, Journals with impact factor
4.7-5.762).
· Monography titled “L-lysine fermentation”. Recent Patents on Biotechnology, Vol. 1,
issue 1,
p. 11-24 (Review article). Request from
Bentham Science Publisher (a
leading international publisher of high quality scientific journals and books; Impact Factor 4.8-5.762).
· Anastassiadis Savas and Morgunov Igor G.
(2007). Gluconic
acid fermentation. Recent
Patents on Biotechnology, Vol. 1, issue 2. Bentham Science Publisher (Review article)
· On January 12, 2007, I received the Nomination Award
as
International Scientist of the Year 2007 (confirmation of nomination on 2nd November 2007). It is nominated to few illustrious individuals whose achievements and
leadership stand out in the International Scientific
Community as decreed by the Research and
Advisory Board sitting at the International Biographical Centre in Cambridge,
England (Outstanding Scientists of the 21st Century and Living Science).
· On January 12, 2007, I received the Outstanding
Scientists of the 21st Century Award from International Biographical Centre in
Cambridge (IBC), England (will include Outstanding Scientists of the 21st Century Diploma and Medal).
· Chosen as candidate and seriously
considered for inclusion in the special 25th Silver Edition of Who’s Who in the World 2007, scheduled to be published in November
2007 (selected).
· Compilation for inclusion in the upcoming 10th Anniversary Edition of Who's Who in Science and Engineering
2008-2009, scheduled to be published in December 2007.
· On February 22, 2007, I attended the International
Conference “EU-Russia
Co-operation:
Priorities for Science & Technology
2007-2013” after
invitation from Directorate of EU (president of biotechnology) and the STATE UNIVERSITY – HIGHER SCHOOL OF ECONOMICS, MOSCOW, RUSSIA.
· Co-Instructor of Diploma thesis and Ph.D. students as Visitor Professor at the Institute of Biochemistry and Physiology
of Microorganisms of the Russian Academy of Sciences
· On March 2, 2007, I received the
Nomination as LEADING
SCIENTISTS of the WORLD 2007 from Awards Board of the IBC in Cambridge, England
(will include Outstanding Scientists of the 21st Century Diploma and Medal). (Ratification on September 28th 2007)
· Named as a Member of the International
Biographical Centre, Cambridge, England, IBC (Nicholas S. Law, Director
General)
· Personal presentations on TV and Radio stations and articles have been written about my scientific
career at several News
Papers.
· On March 30, 2007, I received the
selection to be included in the PRESTIGIOUS PUBLICATION OF “2000 OUTSTANDING INTELLECTUALS OF THE 21st CENTURY” of the International Biographical Centre
in Cambridge (Nicholas S. Law, Director General)
· On 27th April 2007, I received the IBC’s 21st Century Award for Achievement for outstanding contribution from the
International Biographical Centre in Cambridge, England
· On 25th May 2007, I received the IBC’s Archimedes Award for reaching such
goals and being recognized for endeavours both nationally and internationally from the International Biographical Centre in Cambridge, England
· On 8th June 2007, I received the invitation of IBC to be automatically included in the Cambridge
Blue Book of Foremost International Scientists from the International Biographical Centre in Cambridge, England
· On July 20, 2007, I received the Honor
placket of Recognition for the contribution to the Science from the Pontian Association of Chorygi, Kilkis (Greece)
· On July 21, 2007, I received (at the 2nd International Conference of Biotechnology
2007 of Pythia Institut of Biotechnology) a certificate (placket) of official
membership of the RUSSIAN Y.A. OVCHINNIKOV SOCIETY OF
BIOTECHNOLOGISTS from the President, Dr. R. G. Vasilov
· On July 21, 2007, I received (at the 2nd International Conference of Biotechnology
2007 of Pythia Institute of Biotechnology) the Honor certificate (placket) of
congratulations from the
General Director of NTM HOLDING Co.
(Russia) Mr. German E. Karachevkiy (Future joined business and cooperation;
Biodiesel production from plant oils and Joined production of biotechnological
bulk and fine chemicals)
· On July 31, 2007, I
received an invitation
to serve as Guest
Editor and contribute a special topic issue to the exciting journal CURRENT PHARMACEUTICAL BIOTECHNOLOGY, in view of my eminent contributions in the field of pharmaceutical
biotechnology, being as an authority in the field
responsible for soliciting top class reviews from
eminent scientist/researches whom I will invite. The reviews will care various
important aspects of the related topic.
· On 7th December 2007, I received the Nomination of “Top 100 SCIENTISTS 2008” as a
noted and eminent professional in the field of science, from the International Biographical Centre
(IBC) in Cambridge, England and ratification of nomination.
· On 11th January 2008, Inclusion in the new and
revolutionary Biographical Directory:
Outstanding People of the 21st Century, from the IBC in Cambridge, England.
· On 11th January 2008, I have received an
invitation for the selection of 2000 outstanding intellectuals of the 21st century.
· Member of the Society
of Biochemistry and Molecular biology of Russia since 2008 (Moscow society branch).
· On 8th February
2008, I received the decision for inclusion in 2000 OUTSTANDING SCIENTISTS 2008/2009
(Publication in mid 2009),
from the IBC in Cambridge, England.
· Consideration for inclusion in the upcoming 2009 Edition of Who's Who in the World®, which is scheduled for publication in
November 2008.
· February 29th,
Inclusion in the Dictionary
of International Biography 2008 (IBC,
England) (November 28, 2008)
· On 21st March 2008, I received the granting of the
IBC’s 21st Century Award for Achievement
· On 25th April 2008, I received the Ratification of
nomination as Leading
Scientists of the World 2008 (IBC
in Cambridge, England)
· On 25th April 2008, I received the nomination for the
international accolade “International
Professional of the Year 2008”
· On July 11 2008, I received the IBC award FOREMOST PROFESSIONALS of the
WORLD - 2008
· On July 12, I received the award FOREMOST
SCIENTISTS of the WORLD 2008 (IBC)
· On July 25 2008, I received the forwarding
as one of the FOREMOST SCIENTISTS of the World 2008
· On August 8, 2008, I received the
invitation for inclusion in the 2000 OUTSTANDING INTELLECTUALS OF THE 21ST CENTURY
· On August 22 2008, I received the
selection for the inclusion in the IBC 2000 Outstanding Scientists 2008/2009
· November 7, 2008: International Einstein Award for Scientific
Achievement (IBC)
· November 28, 2008, 2011: 2000 Outstanding Intellectuals of the 21st Century – 2009-2010 and 2011
· December 31, 2010, Inclusion in the Dictionary of International Biography 2011 (IBC, England)
· Nomination for “Top 100 Educators 2011” (IBC, England)
· The international Plato Award for Educational Achievement (7th October 2011), (IBC, England)
· Honor award of Recognition for the
contribution to the Science from the Cultural Association of Avgi (Birth place),
Thessaloniki (Greece)
References –
Recommendations
1. Univ. Professor Dr. Hans-Jürgen Rehm
Institute of Molecular Microbiology and Biotechnology
Corrensstrasse 3 – 48149 Münster – Germany
Tel. +49 (251) 83 – 39 820 Fax: +49 (251) 83 – 38 388
E-mail: mibi@uni-muenster.de http://mibi.uni-muenster.de
2. Professor Dr. Christian Wandrey
Director of Institute of Biotechnology 2
Research Center Jülich (Forschungszentrum Jülich GmbH;
former Nuclear
Research Center, KFA)
Leo-Brandt-Strasse - D-52425 Jülich, Germany
Tel. 011-49-2461-613118 Fax. 011-49-2461-613870
E-mail: c.wandrey@fz-juelich.de
3. Bayer Professor Dr. Gregory
Stephanopoulos
Department of Chemical Engineering, Massachusetts Institute of Technology (MIT)
Room 56-469, Cambridge, MA 02139, USA
Tel.: +1-617-253-4583 Fax: +1-617-253-3122, E-mail: gregstep@mit.edu
4. Dr. Igor G. Morgunov
Head of the laboratory of Aerobic
Metabolism of Microorganisms, Institute of Biochemistry and
Physiology of Microorganisms, Russian
Academy of Sciences, 142290, Pushchino, Moscow Region,
Russia). Tel. (095) 9257448 Fax. (095)9563370, Mobile: (007)
-916-77-35-53-1, E-mail:
morgunovs@rambler.ru
Academic Teaching
· Pythia
Institute of Biotechnology (Owner) - Biotechnology and Microbiology - Research
and Teaching (2003-present)
· Co-Instructor of Diploma
thesis and Ph.D. Students as Visitor Professor at the Institute
of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences
(2006-2007) (Dr. Igor G. Morgunov)
· Environmental
Engineering Department of Democritus University of Thrace, (Xanthi, Greece) - Teaching
Environmental
Microbiology, Ecology
(class
and laboratory), Biology (class and laboratory) and Biotechnology (for Ph.D. students) as
Lecturer and Assistant Professor; From October 2000 (C1) to present (C2
Epicurean professor).
· Civil
Engineering Department of Democritus University of Thrace, (Xanthi, Greece) –
Teaching Ecology for Engineers (from Academic year 2010-2011) and exams until
today
· Environmental Engineering
Department of Democritus University of Thrace, (Xanthi, Greece) - Instruction
at the present of more than 20 diploma theses (since 2005)
· Tufts
University (Boston, USA), Instructed students in laboratory
(Biotechnology
Center); 1999
· Massachusetts
Institute of Technology (MIT, Boston, USA), Department of Chemical
Engineering; 3/1994 – 8/1996 - Supervised students in undergraduate research
programs and for Bachelor Thesis and helped Ph.D. students in laboratory (Bayer
Prof. Dr. Gregory Stephanopoulos)
· Research
Center Jülich, RCJ (KFA, former Nuclear Research Center Jülich), Institute for Biotechnology
(Germany); 1990 – 1994 - Supervised Bachelor and Master Students
· University
of Bonn (Germany); 1990 – 1994 - Teaching
Assistant for laboratory courses in biotechnology and technical chemistry
(Prof. Dr. Christian Wandrey)
· University
of Münster (Germany); 1985 – 1989 - Helped to
organize several microbiology courses in the Institute of Microbiology (Prof.
Dr. Hans-Jürgen Rehm)
Special Skills
· Extensive international experiences in Research and
Development and teaching in Fermentation process technology and Industrial and
Classical Microbiology
· Biology, classical and industrial microbiology, biotechnology,
chemical engineering, ecology
· Selective isolation and screening of microorganisms
for industrial applications and innovations
· Classical mutagenesis, analytical methods
· Fermentation technology, development, design and
optimization of biotechnological processes
· Development of continuous fermentation processes
(chemostat) with and without biomass retention
· Utilization of wild type and genetically engineered
microorganisms in batch and continuous cultures
· Cultivation and storage of microorganisms (bacteria,
yeasts, fungi)
· Extensive training in sterile and analytical
techniques (enzymatic, GC, HPLC etc.)
· Extensive fermentation experience with biomass
retention in various types and scales of bioreactors
· Fermentation media and operating parameter
optimization in continuous and batch cultures
· Additional qualification in Reaction Technique,
Biotechnology and Chemical Engineering
· Elucidation of the regulatory mechanisms of microbial
biosynthesis, physiology and pathways
· Technology transfer, cooperation with industrial
partners and patenting
· Academic instruction for undergraduate courses and
Bachelor and Master Theses
· Extensively experienced in laboratory set up, research
organization and design, in starting new projects and in leading a research
group
· Teaching of microbiology, biology, ecology and
biotechnology
· Start up of a Research biotechnology company and of Pythia Institute of
Biotechnology (Greece)
· Starting industrial production of a novel superior
fertilizer at Pythia
Institute of Biotechnology
(Greece)
· International cooperation – Research and Development –
Teaching (Institute
of Biochemistry and
Physiology of Microorganisms of the
Russian Academy of Sciences)
· Initiation of private biotechnology business
(production of novel biological fertilizer)
· Music compositions
Languages: English, German and Greek
Hobbies and other
interests
· Playing piano and keyboards, Music compositions
(copyrighted music in USA and Greece), Text writer.
· Member of Associations (e.g. RUSSIAN Y.A. OVCHINNIKOV SOCIETY OF
BIOTECHNOLOGISTS and Member of the Society of Biochemistry and Molecular biology of
Russia since 2008, Moscow society
branch etc.)
· Honorary member and scientific advisor of the
Association of Biofarmers
of Drama (Greece)
I. Patents,
Conference presentations, Scientific works and Publications
1. ANASTASSIADIS S., AIVASIDIS A. and WANDREY CH.,
1992; "Verfahren zur fermentativen
Gewinnung von Gluconsäure und dafür geeignete
Mikroorganismen", ("Process for the production of
gluconic acid by fermentation and the microorganisms
suitable for the process"). German Patent
Application No. P 42 17 674.3-41 (09/09/1992)
2. ANASTASSIADIS S., AIVASIDIS A. and WANDREY CH.,
1999. "Process for the production of gluconic acid with a strain of Aureobasidium
pullulans (DE
BARY) Arnaud. US
Patent No.
5,962,286 (Oct. 5, 1999)
3. ANASTASSIADIS S., AIVASIDIS A. and WANDREY CH.,
1993; "Fermentationsverfahren zur
kontinuierlichen Citronensäuregewinnung".
(“Process for the continuous production of citric acid by
fermentation”). German patent No. P 43 07 517.7 (03/18/1993)
4. ANASTASSIADIS S., 1994. "Zymotiki methodos gia
tin sinechi paragogi tou kitrikou oxeos”;
(“Process for the continuous production of citric acid
by fermentation”). Greek
Patent No. 940100098 (24.02.1994)
5. ANASTASSIADIS S., AIVASIDIS A. and WANDREY CH.,
1994. "Fermentationsverfahren zur kontinuierlichen
Citronensäuregewinnung" (“Process for the continuous production of citric
acid by fermentation”). Austrian
Patent No. 473/94 (03/07/1994)
6. ANASTASSIADIS S., AIVASIDIS A. and WANDREY CH.,
2001. "Process for the continuous production of citric acid by
fermentation". US
Patent No. 6,303,351 (October
16, 2001)
7. ANASTASSIADIS S., 1998. “Continuous fermentation of
lactic acid” Final
Report for US Patent application (at Thermo Fibergen Inc., a ThermoElectron Co., Boston, USA)
8. ANASTASSIADIS Savas (2007). Production and
composition of an ecological biological plant antifreezing, growth stimulating
and plant protecting fertilizer of microbial origin (Growth stimulating,
protective, antifreezing fertilizer for trees, flowers and other plants). Greek Patent application
(2007). Pythia Institute of Biotechnology
(Avgi-Sohos, Thessaloniki, Greece)
II. International
Conferences
1. ANASTASSIADIS S., AIVASIDIS A. AND WANDREY CH.,
1992. "Continuous Gluconic Acid Fermentation with Yeast-Like Molds".
10th Dechema Meeting of Biotechnologists, June 1 to 3 1992, Karlsruhe , F.R.G
(Poster). Biotechnology Conferences 5-VCH Verlagsgesellschaft 1992, 537-540
2. ANASTASSIADIS S., AIVASIDIS A., WANDREY CH. AND
H.-J. REHM, 1992. "Continuous production of citric acid with yeasts and
gluconic acid with yeast-like molds". VAAM-Tagung`93, Leipzig, Germany
(Conference paper, presentation)
3. SAVAS ANASTASSIADIS, ALEXANDER AIVASIDIS
(Department of Environmental Engineering, Democritus University of Thrace,
GREECE) and CHRISTIAN WANDREY (Institute of Biotechnology of Research Center
Jülich, GERMANY). Continuous citric acid fermentation by Candida oleophila. FINAL
LIST OF POSTERS - BIOCHEMICAL ENGINEERING CONFERENCE - POSTER SESSION I -
Monday, June 11, 2001 (USA)
4. Dr. Savas Anastassiadis (July 23, 2006)
- Organization of the 1nd International Initiation ceremony and
small Conference of Biotechnology of Pythia Institute of Biotechnology (AvgiSohos,
Thessaloniki, Greece) and oral presentation entitled: Future perspectives of Biotechnology
5. Dr. Savas Anastassiadis (December 6-7, 2006) – “Perspectives for microbial production of
gluconic acid and L-lysine” POSTER PRESENTATION AT THE FOURTH CONGRESS OF RUSSIAN Y.A. OVCHINNIKOV SOCIETY OF
BIOTECHNOLOGISTS (Invitation by the
Russian Academy of Sciences) Pushchino
Scientific Centre, Russia
6. On February 22, 2007, I attended the International
Conference "EU-Russia
Co-operation: Priorities for Science & Technology 2007-2013 after invitation from Directorate of EU
(president of
biotechnology) and the STATE UNIVERSITY – HIGHER SCHOOL OF ECONOMICS, MOSCOW, RUSSIA.
7. Dr. Savas Anastassiadis (July 19-21,
2007) – I organized and carried out the 2nd International Conference of Biotechnology
and Alternative Energies of Pythia Institute of Biotechnology
(Avgi Sohos, Thessaloniki, Greece). Oral
presentation entitled: Continuous
production of organic acids (e.g. Citric acid, Gluconic acid, L-Lactic acid,
Succinic acid etc.)” and novel plant protecting agent
(Presence of Science, Politics, Industry and Arts
(Exhibition)): Chairman: Dr. Igor G. Morgunov (RAS)
8. Dr. Savas Anastassiadis (July 19-21,
2007) - Poster presentation entitled: Production of a novel antifreezing, growth and
production promoting and disease protecting fertilizer for plants and trees (Pythia
Institute of Biotechnology). 2nd International Conference of Biotechnology
and Alternative Energies of Pythia Institute of Biotechnology (Avgi Sohos,
Thessaloniki, Greece)
III. Literature
Reviews and Final Research projects and Reports in industry and academy
1. ANASTASIADIS
S., 1989,
“Tempe-Fermentation” (Soybean fermentation), Literature Review work in German, which was translated in English
by the German Government (University of Münster, Germany), page 1-50 Academic supervisor: Prof. Dr. Hans-Jürgen Rehm
2. ANASTASSIADIS Savas and Stephanopoulos Gregory
(1994-1996) Final
Research Report for continuous L-lysine production by coryneform bacteria (MIT,
USA) (100 g/l of L-lysine in chemostat), (Chemical Engineering Department, MIT,
USA) Academic advisor: Prof. Dr.
Gregory Stephanopoulos Autonomous Senior
Scientist
3. ANASTASSIADIS Savas and Stephanopoulos Gregory
(1994-1996) Final
Research Report for the optimization of the effect of betaine on L-lysine
fermentation by coryneform bacteria as a function of glucose concentration
4. ANASTASSIADIS Savas and Stephanopoulos Gregory
(1994-1996) Final
Research Report on the continuous production of citric acid by yeasts (230-250
g/l citric acid in continuous repeated batch in 48-54 h and 120 g/l in repeated
batch cultures) Autonomous Senior Scientist
5. ANASTASSIADIS Savas (1997) Final Research Report on the continuous
production of citric acid by yeasts (200 g/l citric acid in chemostat) (CARGILL, Minneapolis, USA). The project focused on the continuous production of citric acid by
yeast strains of Yarrowia lipolytica (ATCC strains) in chemostat. Autonomous Senior Scientist
6. ANASTASSIADIS S., 1998, “Continuous fermentation of
lactic acid” Final
Research Report for
US Patent application (at Thermo Fibergen Inc., a ThermoElectron
Co., Boston, USA) (150 g/l Llactic
acid) Autonomous Senior Scientist
7. ANASTASSIADIS S., 1998, “Continuous production of a
biodegradable non-corrosive deicer composition” Final Research Report for US Patent
application (at
Thermo Fibergen Inc., a ThermoElectron Co., Boston, USA) (150 g/l L-lactic
acid) Autonomous
Senior Scientist
8. ANASTASSIADIS SAVAS, 2000-2001. Finalization of a
project regarding to the acceleration
of wine fermentation and
writing of a final report in German Language at the Environmental Engineering of Democritus University of Thrace
(Greece). The wine fermentation is completed within 24-30 hours. Autonomous Senior Scientist Academic
advisor: Prof. Dr. Alexander Aivasidis
IV. International
Books
1. Savas Anastassiadis (Pythia Institute
of Biotechnology), Igor G. Morgunov and Tatiana Finogenova (Russian Academy of
Sciences) (2006-2007). Industrial Microbiology and Biotechnology. Review book (Under preparation on Request
from an international publisher) RESEARCH SIGNPOST accepted
2. Anastassiadis Savas, Kamzolova Svetlana
V., Morgunov Igor G. and Rehm Hans-Jürgen (2007). Comparative study of the effect of iron on
Citrate-producing yeast growing on different substrates. Book Chapter at “Communicating Current
Research and Educational Topics and Trends in Applied
Microbiology, FORMATEX
Microbiology series No. 1, Vol. 1 (Book)”
Industrial
and Food Microbiology, p. 308-314, A.
Mendez-Vilas (Ed.). ISBN-13: 978-13:978-84-611-9422-3
3. S.V. Kamzolova, S.G. Anastassiadis2, A.R. Fatyhkova, N.P. Golovchenko, and I.G. Morgunov (2010).
Strain and process development for citric acid. production from glycerol-containing
waste of biodiesel manufacture. FORMATEX Microbiology Book Series No. 2, Vol. 2
(Book), "Current Research, Technology and Education Topics in Applied
Microbiology and Microbial Biotechnology" MICROBIOLOGY BOOK SERIES - Number 2. A. Mendez-Villas (Ed.),
http://www.formatex.info/microbiology2/1020-1028.pdf
4. Anastassiadis Savas (Pythia Institute
of Biotechnology) (2007). Industrial Microbiology. Book Chapter
at Clinical Neuropharmacology (Κλινική Νευροφαρμακολογία - Κεφάλαιο: Βιομηχανική Μικροβιολογία) (Author: Evangelos Dimitriadis, Aristotelis University of Thessaloniki,
Greece), 1st edition, Publisher Siokis
5. Anastassiadis Savas (Pythia Institute
of Biotechnology) (2007). Biotechnology. Book Chapter at Clinical Neuropharmacology
(Κλινική Νευροφαρμακολογία - Κεφάλαιο: Βιοτεχνολογία) (Author:
Evangelos Dimitriadis,
Aristotelis University of Thessaloniki, Greece), 1st edition, Publisher Siokis
6. On July 31, 2007, I
received an invitation
to serve as Guest
Editor and contribute a special topic issue to the exciting journal CURRENT PHARMACEUTICAL BIOTECHNOLOGY, in view of my eminent contributions in the field of
pharmaceutical biotechnology, being as an authority in the
field responsible for soliciting top class reviews
from eminent scientist/researches whom I will invite. The reviews will care
various important aspects of the related topic.
6. Invitation to edit a an encyclopedia (5
books) from Bentham Sciences Publisher
V. International
publications in Journals with reviewers and analytical publications
1. ANASTASSIADIS S., AIVASIDIS A. and WANDREY CH.
(2002). Citric acid production by Candida oleophila under intracellular nitrogen limitation. Applied
Microbiology and Biotechnology 60 (1-2): 81-87 (DOI 10.1007/s00253-002-1098-1, Band 60 (1-2), October 21, 2002
Impact factor: 3.69
2. ANASTASSIADIS S., AIVASIDIS A. and WANDREY CH.
(2003). Continuous gluconic acid production by isolated yeast-like mould
strains of Aureobasidium pullulans.
Applied Microbiology and Biotechnology 61(2): 110-117 (s00253-002-1180-8)
Impact
factor: 3.69
3. Anastassiadis, S., Wandrey, C. and Rehm, H.-J. 2005
(July). Continuous
citric acid fermentation by Candida oleophila under nitrogen limitation at constant C/N
ratio. World Journal of
Microbiology and Biotechnology, Volume 21, Number 5, p. 695-705, ISSN: 0959-3993 (Paper) 1573-0972
(Online), DOI: 10.1007/s11274-004-3850-4 Impact fector: 1.26
4. Anastassiadis, S., Aivasidis, A., Wandrey, C. and
Rehm, H.-J. 2005 (June 2). Process
optimization for continuous gluconic acid fermentation by isolated yeast-like
strains of Aureobasidium
pullulans. Biotechnology
& Bioengineering,
Volume 91, Issue 4, Pages 494-501; PMID:
15937884, Published Online: 2 Jun 2005 Impact factor: 3.65
5. Anastassiadis, S. and Rehm, H.-J. 2005. Continuous citric acid secretion by a high
specific Ph dependent active transport system in yeast Candida
oleophila ATCC
20177. Electronic
Journal of Biotechnology. Vol. 8
No. 2, Issue of August 15, 2005 Impact factor: 0.83
6. Anastassiadis, S. and Rehm, H.-J. 2005. Continuous citric acid secretion by a high
specific pH dependent active transport system in yeast Candida
oleophila ATCC
20177. Electronic
Journal of Biotechnology. Vol. 8
No. 2, Issue of August 15, 2005 BIP Article
7. Anastassiadis
S. Candida citric
acid fermentation. Industrial
Bioprocessing,
Volume 27, Issue 9, September 2005, Pages 6-7
8. Anastassiadis, S. and Rehm, H.-J. 2006. Citric acid production from glucose by
yeast Candida oleophila ATCC 20177 under batch, continuous and repeated batch
cultivation. Electronic Journal of Biotechnology, Vol. 9
No. 1, Issue of January 15, 2006 Impact factor: 0.83
9. Anastassiadis, S. and Rehm, H.-J. 2006. Citric acid production from glucose by
yeast Candida oleophila ATCC 20177 under batch, continuous and repeated batch
cultivation. Electronic Journal of Biotechnology, Vol. 9
No. 1, Issue of January 15, 2006 BIP Article
10. Anastassiadis, S. and Rehm, H.-J. 2006. Oxygen and temperature effect on
continuous citric acid secretion in Candida oleophila. Electronic Journal of Biotechnology, Vol. 9 No. 4, Issue of July 15, 2006
Impact factor: 0.83
11. Anastassiadis, S. and Rehm, H.-J. 2006. Oxygen and temperature effect on
continuous citric acid secretion in Candida oleophila. Electronic Journal of Biotechnology, Vol. 9 No. 4, Issue of July 15, 2006
BIP Article
12. Anastassiadis, S. and Rehm, H.-J. 2006. Continuous gluconic acid production by
Aureobasidium pullulans with and without biomass retention. Electronic Journal
of Biotechnology, Vol. 9 No. 5, Issue of
October 15, 2006 Impact
factor: 0.83
13. Anastassiadis, S. and Rehm, H.-J. 2006. Continuous gluconic acid production by
Aureobasidium pullulans with and without biomass retention. Electronic
Journal of Biotechnology, Vol. 9 No. 5, Issue of October 15, 2006 BIP- Article
14. Anastassiadis, S. and Rehm, H.-J. 2006. Continuous
gluconic acid production in cascading of fermenters. Applied
Microbiology and Biotechnology (accepted
on 14 May 2006, DOI
10.1007/s00253-006-0499-y). Vol. 73(3), p. 541-8. (Epub 2006 Aug 12) Impact factor: 3.69
15. Anastassiadis Savas (2007). L-lysine
fermentation. Recent Patents on Biotechnology, Vol. 1, issue
1, p. 11-24 (Review article). Request from Bentham Science (a leading international publisher of high quality scientific journals and
books; Impact
Factor 4.8-5.762). (ISSN: 1872-2083 - Volume 1, Issue 1, 2007, p. 11-24)
16. Anastassiadis Savas and Morgunov Igor G. (2007). Gluconic acid
production. Recent Patents on Biotechnology, Vol. 1,
Issue 2. Bentham Science Impact Factor 4.8-5.762
17. Anastassiadis Savas, Kamzolova Svetlana V., Morgunov
Igor G. and Rehm Hans-Jürgen (2007). Comparative study of the effect of iron on Citrate-producing
yeast growing on different substrates. Book Chapter at “Communicating Current
Research and Educational Topics and Trends in Applied Microbiology, FORMATEX Microbiology series No. 1, Vol. 1 (Book)” Industrial and Food Microbiology, p. 308-314, A.
Mendez-Vilas (Ed.). ISBN-13: 978-13:978-84-611-9422-3
18. Anastassiadis S. (1993). Institute of
Biotechnology 2, Research Center Jülich (KFA) in Germany."(Application No.
7; "Determination of organic acids, especially citric and isocitric acid,
in fermentation solutions and fruit juices", Published in: Chromatography methods 1993, (Macherey-Nagel GmbH + Co. KG Düren,
Postfach 101352, D-52348 Düren, Germany).
19. Trends in Food Science & Technology 12 (2001)
349–350. New Patents
20. Anastassiadis S. 2007. L-Lysine Fermentation - Patent
Survey Review Article. Feedinfo News Service Scientific Reviews. November 2007.
Available from URL: http://www.feedinfo.com,
Request from Feedinfo News Service
21. Anastassiadis Savas, Kamzolova S.V.,
Morgunov Igor G. and Finogenova Tatiana (2008). Citric acid production patent review. Recent Patents on Biotechnology 2008, Vol.
2(2), 107-123
(Review
article in the patent area of Biotechnology; Request from Recent Patents on Biotechnology, Bentham Science, Impact Factor 4.8-5.762), DOI:
10.2174/187220808784619757, http://www.eurekaselect.com/92714/article
22. Savas Anastassiadis, Svetlana V. Kamzolova, Igor G.
Morgunov, Hans-Jurgen Rehm (2008). Iron effect on gluconic acid production by Aureobasidium
pullulans. The Open Biotechnology Journal, Volume 2 (ISSN: 1874-0707). pp.195-201 (7) 23. S.V. Kamzolova, S.G. Anastassiadis2, A.R. Fatyhkova, N.P. Golovchenko, and I.G. Morgunov (2010). Strain and process development for citric
acid. production from glycerol-containing waste of biodiesel manufacture Microscopy Book Series titled
"Current Research, Technology and Education Topics in Applied Microbiology and Microbial
Biotechnology" A. Mendez-Villas (Ed.),
http://www.formatex.info/microbiology2/1020-1028.pdf
24. Svetlana
V. Kamzolova1*, Alina R. Fatykhova1, Emiliya G. Dedyukhina1, Savas G. Anastassiadis2,
Nikolay P. Golovchenko1 and Igor G. Morgunov1. Citric Acid Production by Yeast Grown
on Glycerol-Containing Waste from Biodiesel Industry. Food Technol. Biotechnol.
49(1) 65–74 (2011) Impact
factor: 0.98
25. Kamzolova Svetlana V., Anastassiadis Savas, Morgunov
Igor G and Finogenova T. (2008) Citric acid production (2007) Mikrobiologia
(Russian), (submitted)
26. E.A. Dimitriadis, S. Anastassiadis, Α. Katranias, D. Mixmizos.
SOURCES OF INTERINDIVIDUAL
VARIATION IN RED BLOOD CELL CHOLINESTERASE ACTIVITY (Laboratory of Neuropathology, A’
Neurologic Clinic, AHEPA, A. U. Th) (under submission)
27. Savas Anastassiadis (2008). Succinic acid
production patent review. Recent
Patents on Biotechnology. Accepted (under preparation)
28. 6 Review articles under preparation (2014)
VI. Publications
under preparation and under submission
1. Anastassiadis
Savas and Kamzolova Svetlana V., Citric acid fermentation by yeasts. Review (under
preparation)
2. Loupa G. and Anastasiadis S. 2006. INDOOR / outdoor PARTICULATE matter and d
bioaerosol levels IN NATURALLY VENTILATED OFFICES (under submission)
3. Anastassiadis,
S. and Rehm, H.-J. 2005. Production of gluconic acid under batch, fed batch and
continuous cultivation (under preparation)
4. Anastassiadis, S. and Rehm, H.-J. 2005. The
phenomena of oscillations in citric acid production by yeast Candida oleophila ATCC 20177. Ovchinnikov bulletin of biotechnology and physical and
chemical biology (English and Russian) (under submission)
5. Savas Anastassiadis1*, Svetlana V. Kamzolova2 and
Igor G. Morgunov2 (2008).
Manganese effect on continuous
gluconic acid production by yeast-like mold Aureobasidium pullulans (under preparation and submission, Applied
Microbiology and Biotechnology)
6. Anastassiadis Savas, Svetlana V. Kamzolova and
Morgunov G. Igor (2007). Succinic
acid production by
various microorganisms, e.g. bacteria and yeasts (under preparation) (Pythia
Institute
of Biotechnology, Democritus University of Thrace and
Institute of Biochemistry and Physiology of Microorganisms of the Russian
Academy of Sciences)
7. ANASTASSIADIS et al. (2007) Several Publications
under preparation (Continuous fermentation of citric acid, gluconic acid,
L-lysine, L-lactic acid, succinic acid etc.)
8. Kamzolova
Svetlana V., Morgunov Igor G., Anastassiadis Savas, and Finogenova Tatiana (2007). Biochemical aspects for citric
acid production in yeasts (Review article)
9. Anastassiadis Savas, Svetlana V.
Kamzolova and Morgunov G. Igor (2007). Pharmaceutical applications of organic
acids (Review article) Under
preparation
VIII. Analytical
chromatography applications (HPLC methods)
1. ANASTASSIADIS S. (1993). Institute of Biotechnology
2, Research Center Jülich (KFA) in Germany. "(Application No. 7;
"Determination of organic acids, especially citric and isocitric acid,
in fermentation solutions and fruit juices", Published in: Chromatography methods 1993, (Macherey-Nagel GmbH + Co. KG Düren,
Postfach 101352, D-52348 Düren, Germany).
2. ANASTASSIADIS S. (1994), Ph.D. thesis, Institute of Biotechnology 2, Research Center Jülich
(KFA) in Germany New HPLC application for selective sugar analysis and
separation of chemically related organic acids
3. ANASTASSIADIS S. (1996-7). ThermoFibergen Inc.
(ThermoElectron Co.), Boston, USA. New HPLC application for selective
separation of organic acids, in especially lactic acid, acetic acid, succinic
acid etc. and sugar analysis (separation of glucose and gluconic acid) (Senior scientist)
Research and
Teaching Interests
I. Classical and Industrial Microbiology
and Biodiversity
The appearance of carbohydrate-based microbial
consumer products is a recent phenomenon. The vast majority of these products,
including commodity, specialty and fine chemicals (e.g. carbonic acids such as
citric, gluconic, lactic and itaconic acid, ethanol, pharmaceuticals, vitamins,
recombinant proteins, enzymes, precursors for polymers, biodegradable plastics
etc.) clearly demonstrate the
tremendous economic potential for future
biotechnological applications. The contribution of microorganisms to
biotechnology is essential. The full potential of microorganisms to industrial applications
is still not quantified. The isolation and screening of wild microorganisms and
mutant strains, including bacteria, yeasts and fungi can be performed to assess
their potential. Biotechnology will have a critical impact in the world’s
economy and in solving future world problems encompassing
environment, agriculture, health and energy. In order
to quantify the full potential of microbial organisms, I am interested in:
· The isolation, characterization and use of microbial
wild and mutant strains of industrial importance
· The production of important bulk, fine and specialty
chemicals economically
II. Fermentation process development and
optimization
Important factors in improving competitiveness of
biological vs. chemical systems are yield and productivity enhancement.
Sophisticated optimization of environmental process variables can have a tremendous
improvement on fermentation processes. This was clearly illustrated in my work
on the continuous production of citric and gluconic acid, where improvements of
10- to 20-fold were achieved
and in my work on the continuous fermentation of
L-lysine, succinic acid, L-lactic acid and novel fertilizer. In addition, the
developed continuous processes have many advantages over the traditional batch
and fed-batch operations of the last 100 years.
As a result, I am interested in:
· Analyzing and optimizing existing fermentation
processes and introducing new processes
· Studying the specific use of various carbon sources,
including cheap agricultural wastes to produce specialty products such as
carbonic acids, amino acids, food additives, fine chemicals and pharmaceuticals
· Quantifying the use of various feeding strategies in
fed-batch fermentations to simulate continuous chemostat operation.
III. Elucidation of microbial physiology
and regulation
The understanding of microbial physiology and
regulation plays an important role in redirecting intermediary cellular
metabolism to desirable products. Enzyme regulation and product secretion can
be influenced by environmental factors such as trace elements, growth factors
as well as by physical parameters. For example, iron-limiting yeast cultures
overproduced citric acid, while iron sufficient
cultures primarily formed biomass, as a result of
aconitase regulation.
Due to the complexity of living systems (a typical
bacterial cell includes more than 1000 enzymes) it is essential to identify
critical aspects of metabolite overproduction. This requires an insightful and systematic
approach.
As a result, I am interested in studying the:
· Clarification of interactions between enzyme
reactions, pathways and product secretion
· Use of auxiliary carbon sources to increase the
productivity
· Ways to achieve maximum microbial production capacity
by manipulating the environmental parameters and physiology
· Combination of continuous and batch fermentation to
use optimal conditions from both types
· Selective isolation of high-producing mutant strains
Furthermore, I am interested in starting biotechnology
business for the production of biotechnological products and technology
transfer.
IV. Teaching (Class and laboratory)
Biology, Microbiology
(Classical, Environmental
and Industrial Microbiology), Ecology and Microbial
Ecology and Biotechnology
(Environmental microbiology, Fermentation technology and
Process development, Isolation and screening of microorganisms etc.)
Current Research and
Development (Academy and Pythia Institute of Biotechnology Research Center,
Cooperation with the Institute of Microbiology of the RAS)
· Research and Teaching at the Environmental
Engineering Department of Democritus University of Thrace (Xanthi, Greece)
· Instruction of several diploma theses
(currently about 20 diploma thesis students)
· Completed a Greek-German research project
regarding the acceleration of most fermentation using Genetic Algorithms
(Computer program). Glucose was completely consumed and wine fermentation
accomplished within 24-30 hours, compared with more than 6 days that is necessary
for wild most.
· Fermentation technology (Process
development and optimization) and Waste minimization during the industrial
production of biotechnological products
Citric acid production from various
agro-industrial waste sources,
Gluconic acid from glucose and glucose
containing sources,
Succinic acid production from various
agro-industrial waste sources,
Lactic acid production L-Lysine
fermentation
Product separation and purification
(Classical calcium hydroxide/acid separation, Ion
exchange chromatography)
Use of agro-waste byproducts such as
molasses, glycerol etc.
Isolation of new important microorganisms
from natural ecosystems
Strain development by classical
mutagenesis, selection and screening of industrial
microorganisms
· Atmospheric indoor and outdoor microbial
pollution (methodology for rapid determination)
· Anticancer activities (Understanding and
searching for cancer treatment; Involvement of microorganisms; Changing
metabolism etc.)
Note: My Research Center, Pythia Institute of Biotechnology is well equipped fulfilling international standards
and it possesses new industrially important technologies. It has cooperation
with the Institute of Biochemistry and Physiology of Microorganisms of the
Russian Academy of Sciences. It plans to
organize special summer courses for Russian and EU
students on classical and industrial microbiology, fermentation technology and
process development.
Research Associate at Tufts University
(Boston, USA)
Writing proposals for grants and research on the
microbial production of wild and novel biopolymers by wild and mutant microbial
strains (emulsan, pullulan, cellulose etc), as well as on genetics. Several
interesting mutant microbial strains were developed.
Postdoctoral Research at MIT (Boston, USA)
The primary scope of this work was to study and understand
the formation of amino acids in Corynebacteria. New processes were developed and optimized for the
continuous production of Llysine.
Regulatory and auxotrophic mutant strains of Corynebacterium
glutamicum and Brevibacterium lactofermentum
(ATCC strains) were used.
I. Studied the effects of fermentation parameters,
such as pH, dissolved oxygen, agitation, temperature and media composition on
process yield and productivity. 65 g/l of L-lysine have been achieved and maintained for long period of at residence
times of 25 to 27 hrs. This concentration corresponds to 2.6 g/(l h) volumetric
productivity or 0.1 g/(g h) specific productivity. About 40 g/l of L-lysine
have also
been achieved at a residence time of 14 hours, which
corresponds to a volumetric productivity of 2.9 g/l h. Process yields
approached 45% and were maintained at over 30% g/g at steady state and 100% conversion.
These results compare very favorably with industrial fed-batch processes
employing high production strains. Continuous production has been maintained
for more than 70 days.
II. Isolated high lysine production strains from
chemostat experiments, and characterized them in shake flask and chemostat
cultures along with ATCC strains. Faster growing strains have been isolated
that can reduce operation time in shake flasks by a factor of 4.
III. Studied effects of osmoprotectans, such as
glycine-betaine on growth and lysine production in batch cultures of Corynebacteria (12 l fermenter) at various initial
glucose concentrations. Optimum betaine concentrations were found to reduce
significantly lag phases, especially at high glucose concentrations
and also to improve lysine production.
IV. Isolated yeast-like molds of Aureobasidium
pullulans from
wild flowers (Massachusetts) which produce gluconic acid and were studied in
batch and chemostat experiments. More than 200 g/l of gluconic acid was
obtained in batch experiments.
V. Preliminary development of fermentation process for
the continuous production of trehalose (Disaccharide, 200 Dollar/Kg) by Corynebacteria. Experimental results indicated a
feasible large-scale process. Trehalose can be used in order to keep dried food
fresh after rehydration and in pharmaceuticals.
VI. Developed various new processes for the
semicontinuous production of citric acid using Candida strains. Citric acid concentrations of 240 g/l have
been obtained in fed-batch and repeated fed-batch operation (12 l fermenter)
within 140 hrs using different feeding strategies.
The process yield was more than 70% at 100% conversion
of glucose. This operation was maintained for a period of more than 2 months.
Maximum volumetric productivities between 5 to 6 g/(l h) and specific
productivities between 0.1 to 0.15 g/(g h) were achieved. Numerous repeated
batch fermentations were performed with different Candida strains from ATCC in 1 l fermenters. 110 g/l of citric
acid were produced repeatedly batch-wise in 42-hrs total fermentation time.
Ph.D. Thesis
The primary task of my Ph.D. Thesis was to isolate and
characterize microbial systems suitable for the continuous production of
carboxylic acids of low molecular weight.
I. Isolated about 500 carbonic acid producing strains
from natural ecosystems and compared them with existing citric acid producing
yeast strains. The isolated yeast-like molds of Aureobasidium pullulans were found to be ideal for gluconic acid
production.
II. Οptimized both the growth media composition (iron,
manganese, copper, zinc, magnesium, phosphate etc.) for the above strains, as
well as the process conditions (pH, temperature, dissolved oxygen, pressure and
CO2). Competition effects between growth and
production were determined in continuous cultures.
Process optimization resulted in 1000% improvement for
citric and gluconic acid production in chemostat. Various types of bioreactors
such as stirred and fluidized bed fermenters, single and series vessels with
and without biomass retention by microfiltration and immobilization were
evaluated for their applicability in these processes. Furthermore,
electrodialysis was tested for the separation of citric
acid. This work resulted in two new continuous
processes with high process yields and productivity, using free growing wild
type cells. These new yeast-based continuous processes are highly advantageous
compared to the traditional fungi-based processes of the last 100 years. Due to
the industrial importance of these processes, numerous patent applications have
been submitted (see enclosure).
III. Achieved 150 g/l of citric acid in continuous
culture at residence times of 80 to 100 hrs with Candida oleophila (Yarrowia lipolytica according to new classifications). The
maximum process yield was 75% g/g. Maximum volumetric productivities of 2 g/l h or
biomass-specific productivities of 0.1 g/g h have been determined. In repeated batch fermentations 170
g/l of citric acid have been obtained.
IV. Overproduced 370 g/l of gluconic acid with
isolated strains of Aureobasidium pullulans in continuous processes at residence times of 20 to 25 hrs with molar
process yields of more than 95% (100% g/g). Maximum volumetric productivities
of 20 g/l h or biomass-specific productivities of 10 g/g h were achieved. Even
higher gluconic acid concentrations (500 g/l) have been attained in fed-batch
mode.
V. Illustrated for the first time that citrate
secretion in yeast occurs by an energy consuming transport system, which is
specific for citrate and not isocitrate. Developed a working hypothesis about
the overproduction of citric acid in yeast that is linked with the proposed
transport system and the yeast’s
physiology.
VI. Developed new HPLC methodologies to separate and
quantify citric and isocitric acid or glucose and gluconic acid.
Master Thesis
(Diplomarbeit)
Completed many curriculum courses in classical and
applied Microbiology, Biology and special courses in Biochemistry, Molecular
Biology, Genetics and Immunology.
Composed a literature review on the solid-state
fermentation of soybeans (Tempe) for a German- Indonesian scientific
cooperation.
The objective of my Master Thesis was to study the
microbial events that take place during the soaking of soybeans. Briefly, these
include an initial acidification step followed by a solid-state fermentation
using soybeans (Tempe
or Tempeh). Tempe
is a broadly used vitamin-rich meatanalogous food in Indonesia.
I. Isolated hundreds of bacteria strains from various
kinds of soybeans (Indonesia, China, USA and Brazil) and from soaking water
(from Indonesia). These strains mostly included lactic acid bacteria, and were lyophilized for long-term
studies.
II. Screened the isolated strains for the
acidification of soybeans during the soaking process. Some isolated strains
acidified the soaking water to pH 4.5 within 4 to 5 hrs, compared with the
traditional Indonesian soaking process that requires 24 hrs.
III. Analyzed spontaneous fermentation byproducts
resulting from the soaking of soybeans such as sugars, proteins and vitamins
and spontaneous acidification.
IV. Screened the isolated strains for their ability to
produce various essential
vitamins e.g. B1, B6, B12 in order to increase the nutritional value of Tempe.
Vitamins were analyzed using auxotrophic mutant strains.
Current Research
activities at the Environmental Engineering Department of Democritus University
of Thrace
· Microbiological pollution of Indoor and Outdoor
atmospheric environments
· Process development, process optimization and
Minimization of waste materials during the industrial production of
biotechnological bulk products, e.g. Citric acid (250 g/l), Gluconic acid (430 g/l
in chemostat, 504 g/l in fed batch), Lactic acid (150 g/l), L-lysine (90 g/l),
Pullulan, Biodiesel, Bioethanol, Hydrogen etc.
· Biotechnological production of bulk chemicals using
byproducts of industry, e.g. glycerol, plant and animal oils etc.
· Biotechnological production of bulk chemical using
renewable carbon sources such as plant and animal oils etc.
Current Research activities at the
Biotechnology Research Center (Avgi/Sohos-Thessaloniki)
· Continuous production of citric acid by yeasts
(200-250 g/l)
· Continuous citric acid production of gluconic cid by
yeast-like molds (430 g/l)
· Continuous production of lactic acid (150 g/l)
· Continuous production of succinic acid by bacteria
(more than 40 g/l)
· Continuous production of an ecological non-corrosive
biodegradable deicer (140 g/l)
· Continuous production of pullulan by yeast-like molds Aureobasidium
pullulans
· Continuous production of L-lysine by Corynebacterium strains
· I developed at Pythia Institute of Biotechnology a new
process for the production of a novel microbial fermentation product mixture
(biodegradable, ecological, nontoxic etc.), which protects the plants and trees
from freezing and enhances their growth and production. It also functions against
plant pathogenic fungi, bacteria and insects; I started the commercial
production of the new product.
Reviewer of research
articles at leading international journals
1. Biotechnology & Bioengineering
2. Chemical Papers
Prof. Milan Polakovic, Editor-in-Chief, Chemical
Papers
E-mail: chempap@savba.sk
3. International Journal of Food
Properties (IJFP)
Shyam S. Sablani, Ph.D., Associate Professor of Food
& Bioprocess Engineering
Sultan Qaboos University, P.O. Box-34, Al-Khod 123,
Muscat, Oman
Tel: +968 2414 1289; Fax: +968 244 13 418
E-mail: shyam@squ.edu.om Email: sablanis@yahoo.com
4. Applied Biochemistry and Biotechnology
E-mail: adani@engr.ucr.edu
5. Current Microbiology (Springer)
Erko Stackebrandt, Current Microbiology Editorial
Office, E-mail:
curr.microbiol@dsmz.de
Dr. Tom Edrington, Associate Editor, Current
Microbiology, E-mail:
edrington@ffsru.usda.gov
Dr. Ashvini Chauhan, Associate Editor, Current
Microbiology, E-Mail:
ashvini.chauhan@famu.edu
6. Recent Patents on Biotechnology (BIOT)
Bentham Science Publishers Ltd. (Impact Factor 4.8-5.762)
Ms. Tayyaba Zaidi, Manager Publications, E-mail: biot@bentham.org
Dr. Khurshid Zaman, Editor
Fax: (+92-21)-4960510, Email: patents@bentham.org, URL: www.bentham.org/biot
7. Journal Open Biotechnology
Bentham Science Publishers Ltd. (Impact Factor 4.8-5.762)
Kazim Ali Baig Manager Publications ambreen Shakeel
<ambreenshakeel@bentham-science.org>,
tobiotj@bentham.org, obiotl@bentham.org, obiotr@bentham.org
8.
Current Pharmaceutical Biotechnology (Bentham Science Publishers Ltd.)
On July 31, 2007, I received an invitation to serve as Guest Editor and contribute a special topic issue to the exciting journal CURRENT PHARMACEUTICAL
BIOTECHNOLOGY, in view of my eminent contributions in the field of pharmaceutical biotechnology,
being as an authority in the field responsible for soliciting top class reviews
from eminent scientist/researches whom I will invite. The reviews will care various
important aspects of the related topic.
Dr. Alain P. Rolland, Vice President
Editor-in-Chief, Vice President, Product Development
Vical, Inc., 10390 Pacific Center Court San Diego, CA
92121, 92121, United States, E-mail: cpb@bentham.org
9. Revista Latinoamericana de MICROBIOLOGÍA Brenda
Valderrama, Ph.D., Editor
10.
FORMATEX (Spain) Journal of Industrial Microbiology & Biotechnology
